The province reported 29 new cases of monkeypox on Tuesday, with labs reporting a total positivity rate of nearly one in three since May.
The province now has 478 confirmed cases, up from 449 last week.
Among the confirmed cases, 75% (359) are in Toronto residents.
Fifteen people have required hospitalization since May, and two people have required treatment in an intensive care unit.
Seven other suspected cases are still under investigation.
Public Health Ontario told CP24 on Wednesday that as of Monday, 1,636 monkeypox tests had been completed in the province, yielding a positivity rate at the time of 31.6 per cent.
There are now three confirmed cases in women, up from two last week, still accounting for just 0.6 percent of all known cases.
Other areas of the province account for the remaining 25% of the caseload, with Ottawa (36 cases), Middlesex-London (13 cases), Hamilton (11 cases) and Halton and Durham (9 cases each) leading the count among others places.
The most common symptoms continue to be skin rashes, oral/genital lesions, fever and fatigue.
Earlier this week, Ontario Chief Medical Officer Dr. Kieran Moore said 20,000 Ontarians have so far received the Imvamune smallpox and monkeypox vaccine, and that the vaccination effort it helped slow down the transmission of the virus.
The City of Toronto continues to host vaccination clinics targeting the most vulnerable communities.
The US Centers for Disease Control says it is aware of more than 30,000 confirmed cases of monkeypox in 88 countries around the world.
The Public Health Agency of Canada says it is aware of 957 cases of monkeypox across Canada.
The virus is spread primarily through close and sustained contact via respiratory droplets, direct skin-to-skin contact, or contact with contaminated clothing or bedding.